Histopathological Injuries, Ultrastructural Changes, and Depressed TLR Expression in the Small Intestine of Broiler Chickens with Aflatoxin B₁.

نویسندگان

  • Fengyuan Wang
  • Zhicai Zuo
  • Kejie Chen
  • Caixia Gao
  • Zhuangzhi Yang
  • Song Zhao
  • Jianzhen Li
  • Hetao Song
  • Xi Peng
  • Jing Fang
  • Hengmin Cui
  • Ping Ouyang
  • Yi Zhou
  • Gang Shu
  • Bo Jing
چکیده

To explore AFB₁-induced damage of the small intestine, the changes in structure and expression of TLRs (Toll-like Receptors) in the small intestine of chickens were systematically investigated. Ninety healthy neonatal Cobb chickens were randomized into a control group (0 mg/kg AFB₁) and an AFB₁ group (0.6 mg/kg AFB₁). The crypt depth of the small intestine in the AFB₁ group was significantly increased in comparison to the control chickens, while the villus height and area were evidently decreased, as well as the villus:crypt ratio and epithelial thickness. The histopathological observations showed that the villi of the small intestine exposed to AFB₁ were obviously shedding. Based on ultrastructural observation, the absorptive cells of small intestine in the AFB₁ group exhibited fewer microvilli, mitochondrial vacuolation and the disappearance of mitochondrial cristae, and junctional complexes as well as terminal web. Moreover, the number of goblet cells in the small intestine in the AFB₁ group significantly decreased. Also, AFB₁ evidently decreased the mRNA expression of TLR2-2, TLR4, and TLR7 in the small intestine. Taken together, our study indicated that dietary 0.6 mg/kg AFB₁ could induce histopathological injuries and ultrastructural changes, and depress levels of TLR mRNA in the chicken small intestine.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Effects of Dietary Conjugated Linoleic Acid and n-3 Fatty Acids on the Performance, Carcass Traits and Small Intestinal Morphology of Broiler Chickens

An experiment was conducted on broiler chickens to study the effects of conjugated linoleic acid (CLA), fish oil, both at 7% of the diet, or their mixtures at 3.5% of the diet, on the performance, carcass traits and intestinal morphology of broiler chickens. The chicks fed with 7% fish oil or 7% CLA diets, were found to have an inferior weight gain in grower and finisher phases, respectively. A...

متن کامل

Effects of Different Levels of Raw and Processed Oak Acorn (Quercus castaneifolia) on Performance, Small Intestine Morphology, Ileal Digestibility of Nutrients, Carcass Characteristics and Some Blood Parameters in Broiler Chickens

This study was conducted to determine the effect of oak acorn (Quercus castaneifolia) on performance, small intestine morphology, ileal digestibility of nutrient, carcass and some blood parameters in broiler chicks. A total of fifty hundred four one-d old male chickens (Ross308 strain) with seven treatments and four replicates in each treatment were used. Experimental diets were: control (corn-...

متن کامل

Effect of milk thistle on the immune system, intestinal related variables, appearance and mortality of broilers contaminated with Aflatoxin B1

Background & Aim: Aflatoxin (AF) is the most important fungus that contaminates food and feed and can enter the food chain of humans. The aim of this study was to investigate the ability of thistle seeds (silybum marianum L.) to reduce the adverse effects of aflatoxin B1 (AFB1) on the immune system, variables related to the intestine, appearance and mortality of t...

متن کامل

Effect of milk thistle on the immune system, intestinal related variables, appearance and mortality of broilers contaminated with Aflatoxin B1

Background & Aim: Aflatoxin (AF) is the most important fungus that contaminates food and feed and can enter the food chain of humans. The aim of this study was to investigate the ability of thistle seeds (silybum marianum L.) to reduce the adverse effects of aflatoxin B1 (AFB1) on the immune system, variables related to the intestine, appearance and mortality of t...

متن کامل

Histomorphometrical Study of the Prebiotic Effects on Intestine Morphology and Growth Performance of Broiler Chickens

This experiment was conducted to compare the effects of prebiotic as alternative feed additive to an antibiotic growth promoter (bacitracin methylene disalicyate) on the growth performance and morphometrical parameters of the small intestine of broiler chickens. One hundred and forty four day old broiler chicks were randomly assigned to one of three dietary treatments for 6 wk and each treatmen...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Toxins

دوره 10 4  شماره 

صفحات  -

تاریخ انتشار 2018